LABORATORY MEDICINE AND CLINCAL BIOCHEMISTRY
Academic year and teacher
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- Versione italiana
- Academic year
- 2022/2023
- Teacher
- CARLO CERVELLATI
- Credits
- 12
- Didactic period
- Primo Semestre
Training objectives
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Modulo: 56370 - BIOCHIMICA CLINICA
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Clinical Biochemistry aims to give to the student the capacity of using and translating the information and data provided by Laboratory Medicine in clinical practice, i.e. screening, diagnosis, prognosis, and monitoring of therapeutic efficacy. Laboratory Medicine aims to give the tools for tissue engineering and regenerative medicine.¿¿
Knowledge and understanding:¿¿
At the end of the course the student will:¿
- be able to interpret and apply in clinical practice the data generated by clinical biochemistry labs, regarding renal and hepatic functionality, electrolytes, markers of inflammation, markers of lipid and carbohydrate metabolism.¿
- be able to use the quantitative information present in a laboratory analysis report in a clinically useful “tool”¿
- able to select the right biomaterials, print tissue or organs on 3D
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Modulo: 69518 - MEDICINA DI LABORATORIO
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Clinical Biochemistry aims to give to the student the capacity of using and translating the information and data provided by Laboratory Medicine in clinical practice, i.e. screening, diagnosis, prognosis, and monitoring of therapeutic efficacy. Laboratory Medicine aims to give the tools for tissue engineering and regenerative medicine.¿¿
Knowledge and understanding:¿¿
At the end of the course the student will:¿
- be able to interpret and apply in clinical practice the data generated by clinical biochemistry labs, regarding renal and hepatic functionality, electrolytes, markers of inflammation, markers of lipid and carbohydrate metabolism.¿
- be able to use the quantitative information present in a laboratory analysis report in a clinically useful “tool”¿
- able to select the right biomaterials, print tissue or organs on 3D Prerequisites
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Modulo: 56370 - BIOCHIMICA CLINICA
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CLINICAL BIOCHEMISTRY¿Knowledge of chemistry, biochemistry, molecular biology, human physiology and anatomy¿¿
LABORATORY MEDICINE¿Knowledge of chemistry, biochemistry, molecular biology, human physiology and anatomy
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Modulo: 69518 - MEDICINA DI LABORATORIO
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CLINICAL BIOCHEMISTRY¿Knowledge of chemistry, biochemistry, molecular biology, human physiology and anatomy¿¿
LABORATORY MEDICINE¿Knowledge of chemistry, biochemistry, molecular biology, human physiology and anatomy Course programme
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Modulo: 56370 - BIOCHIMICA CLINICA
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CLINICAL BIOCHEMISTRY¿
- Plasma proteins: normal proteinaemia and quantitative/qualitative alterations, role of major plasma proteins, albumin and globulins, antibodies, monoclonal gammopathies, acute phase proteins, tumor markers¿
- Water and electrolytes: control of water and electrolyte metabolism, states of dehydration, water intoxication. Sodium hypo- and hypernatremia. Potassium, hypo-and hyperkalemia..¿
- Calcium metabolism and regulation: physiological roles of calcium, control of serum calcium and endocrine-related effects, metabolism of bone tissue.¿
- Copper and iron: physiological role of Cu and Fe, diseases related to overload and deficiency (Wilson's disease, iron deficiency hemochromatosis).¿
- Clinical enzymology: plasma "resident" enzymes and markers of tissue damage. Diagnostic utility in heart ,muscle, liver, pancreas, bone, kidney and prostate diseases.¿
- Disorders of lipid metabolism: plasma transport of lipids and lipoproteins; physiopathology of primary and secondary lipid disorders. Diagnosis of dyslipidemia in prevention of atherosclerotic disease.¿
- Metabolism of carbohydrates: diabetes mellitus, functions of endocrine pancreas and insulin deficiency and periphery resistance in type I and type II DM; alterations of blood glucose, glycosuria. Oral, intravenous and tolbutamide tolerance test. Glycated proteins.¿
- Liver disease: physiological functions of liver; cholestatic and cytotoxic liver diseases, acute and chronic liver diseases, markers of liver injury, bilirubin metabolism and jaundice.¿
- Diseases of the kidney and urinary system. Laboratory test of renal function; Glomerulal and tubular diseases. Acute and chronic renal failure; urine testing, proteinuria and haematuria.¿¿
LABORATORY MEDICINE¿
-Cell cultures, (cell lines, primary cultures, stem cell isolation from tissue, amplification, counting, freezing) video on cell isolation, amplification, etc..
¿-Biobanks (what are they, legislation, applications);¿
-Exosomes (definition, classification, isolation methodologies, characterization, applications) (video isolation exosomes);¿
-Morphological analysis of samples: microscopy: inverted microscopy, confocal microscope, electron microscopy (TEM, SEM) (video: inclusion of samples, cutting, staining).¿
-Cell Analysis: live/dead analysis, cell proliferation, biocompatibility, cytotoxicity, cytofluorimeter, apoptosis test, necrosis, autophagy (Video: biocompatibility test)¿
-Protein analysis: Elisa, western blotting (Video Elisa/ western blotting)¿
-Nucleic acid analysis: DNA-RNA quantification, miRNA, cytogenetics¿Microbiology: biofilms¿-Biomaterials and Tissue Engineering (biomaterials, in vitro reconstruction of bone, leather)¿-3D printers (classification, applications)¿
- Regenerative Medicine
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Modulo: 69518 - MEDICINA DI LABORATORIO
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CLINICAL BIOCHEMISTRY¿
- Plasma proteins: normal proteinaemia and quantitative/qualitative alterations, role of major plasma proteins, albumin and globulins, antibodies, monoclonal gammopathies, acute phase proteins, tumor markers¿
- Water and electrolytes: control of water and electrolyte metabolism, states of dehydration, water intoxication. Sodium hypo- and hypernatremia. Potassium, hypo-and hyperkalemia..¿
- Calcium metabolism and regulation: physiological roles of calcium, control of serum calcium and endocrine-related effects, metabolism of bone tissue.¿
- Copper and iron: physiological role of Cu and Fe, diseases related to overload and deficiency (Wilson's disease, iron deficiency hemochromatosis).¿
- Clinical enzymology: plasma "resident" enzymes and markers of tissue damage. Diagnostic utility in heart ,muscle, liver, pancreas, bone, kidney and prostate diseases.¿
- Disorders of lipid metabolism: plasma transport of lipids and lipoproteins; physiopathology of primary and secondary lipid disorders. Diagnosis of dyslipidemia in prevention of atherosclerotic disease.¿
- Metabolism of carbohydrates: diabetes mellitus, functions of endocrine pancreas and insulin deficiency and periphery resistance in type I and type II DM; alterations of blood glucose, glycosuria. Oral, intravenous and tolbutamide tolerance test. Glycated proteins.¿
- Liver disease: physiological functions of liver; cholestatic and cytotoxic liver diseases, acute and chronic liver diseases, markers of liver injury, bilirubin metabolism and jaundice.¿
- Diseases of the kidney and urinary system. Laboratory test of renal function; Glomerulal and tubular diseases. Acute and chronic renal failure; urine testing, proteinuria and haematuria.¿¿
LABORATORY MEDICINE¿
-Cell cultures, (cell lines, primary cultures, stem cell isolation from tissue, amplification, counting, freezing) video on cell isolation, amplification, etc..
¿-Biobanks (what are they, legislation, applications);¿
-Exosomes (definition, classification, isolation methodologies, characterization, applications) (video isolation exosomes);¿
-Morphological analysis of samples: microscopy: inverted microscopy, confocal microscope, electron microscopy (TEM, SEM) (video: inclusion of samples, cutting, staining).¿
-Cell Analysis: live/dead analysis, cell proliferation, biocompatibility, cytotoxicity, cytofluorimeter, apoptosis test, necrosis, autophagy (Video: biocompatibility test)¿
-Protein analysis: Elisa, western blotting (Video Elisa/ western blotting)¿
-Nucleic acid analysis: DNA-RNA quantification, miRNA, cytogenetics¿Microbiology: biofilms¿-Biomaterials and Tissue Engineering (biomaterials, in vitro reconstruction of bone, leather)¿-3D printers (classification, applications)¿
- Regenerative Medicine Didactic methods
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Modulo: 56370 - BIOCHIMICA CLINICA
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CLINICAL BIOCHEMISTRY¿
The course provides frontal lectures and on-line assisted teaching. The on-line teaching hours of moodle platform certification service consist in the insights of some topics presented during the frontal lessons and indicated by the teacher at the beginning of the course¿¿
LABORATORY MEDICINE¿
The course provides frontal lectures and on-line assisted teaching. The on-line teaching hours of moodle platform certification service consist in the insights of some topics presented during the frontal lessons and indicated by the teacher at the beginning of the course
------------------------------------------------------------
Modulo: 69518 - MEDICINA DI LABORATORIO
------------------------------------------------------------
CLINICAL BIOCHEMISTRY¿
The course provides frontal lectures and on-line assisted teaching. The on-line teaching hours of moodle platform certification service consist in the insights of some topics presented during the frontal lessons and indicated by the teacher at the beginning of the course¿¿
LABORATORY MEDICINE¿
The course provides frontal lectures and on-line assisted teaching. The on-line teaching hours of moodle platform certification service consist in the insights of some topics presented during the frontal lessons and indicated by the teacher at the beginning of the course Learning assessment procedures
- CLINICAL BIOCHEMISTRY¿The learning verification will be carried out by a written exam with a duration of 40minutes. No teacher’s handouts, personal notes or books are allowed. The test will be formulated with multiple-choice questions (5 options per question with only one correct) relating to all the topics covered in class and in order to verify the knowledge described in the teaching program/objectives. Each question will be marked with a maximum of 1 point and the test will be passed with a minimum of 25 out of 43 points. The maximum score is 30 with lode (reached with more than 40 correct answers).
LABORATORY MEDICINE¿The objective of the examination test is to verify the level of achievement of the training objectives indicated above. The examination will consist of a written test. The assessment is expressed in thirtieths (minimum mark 18). The written test includes 43 questions relating to the main topics covered in the course. Each question will be marked with a maximum of 1 point and the test will be passed with a minimum of 25 out of 43 points. The maximum score is 30 with lode (reached with more than 40 correct answers).¿¿
The final grade of the course will result from the means of the grade obtained in Clinical Biochemistry and Laboratory Medicine Reference texts
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Modulo: 56370 - BIOCHIMICA CLINICA
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Marshall, Bangert, Clinical Chemistry, Mosby¿
Antonozzi I, Gulletta E. Medicina di Laboratorio, Logica & Patologia Clinica, Piccin Ed¿¿
Files with lecture slides will be also provided by the lecturer to the students
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Modulo: 69518 - MEDICINA DI LABORATORIO
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Marshall, Bangert, Clinical Chemistry, Mosby¿
Antonozzi I, Gulletta E. Medicina di Laboratorio, Logica & Patologia Clinica, Piccin Ed¿¿
Files with lecture slides will be also provided by the lecturer to the students
