IMMUNOLOGY AND IMMUNOHEMATOLOGY
Academic year and teacher
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- Versione italiana
- Academic year
- 2022/2023
- Teacher
- GIAN MATTEO RIGOLIN
- Credits
- 6
- Didactic period
- Secondo Semestre
Training objectives
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Modulo: 012936/1 - IMMUNOLOGIA
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IMMUNOLOGY
To give basic knowledge on the immune system and the defense against pathogens; deepening: phagocytosis, antibody response, the complement, self-non self recognition, transplant rejection, pathologies due to malfunction immune system for the correct application of these concepts to the laboratory diagnostic proceed.
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Modulo: 020135/1 - EMATOLOGIA
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HEMATOLOGY
Learning of the theoretical notions and techniques useful for the recognition, treatment and monitoring of the main haematological diseases.
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Modulo: 53753 - TECNICHE DI IMMUNOEMATOLOGIA
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TECHNIQUES OF IMMUNOHEMATOLOGY
The student will learn the fundamental aspects of the principal laboratory techniques in immunohematology and hematology in the optics of the application to tranfusional RBC typization and in study of hemolytic anemia. Skills with main techniques of trasfusional medicine. Prerequisites
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Modulo: 012936/1 - IMMUNOLOGIA
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Knowledge of biology, genetics, molecular biology, chemistry, biochemistry
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Modulo: 020135/1 - EMATOLOGIA
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Knowledge of biology, general pathology, genetics, molecular biology, biochemistry
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Modulo: 53753 - TECNICHE DI IMMUNOEMATOLOGIA
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Knowledge of biology, genetics, molecular biology, chemistry, biochemistry Course programme
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Modulo: 012936/1 - IMMUNOLOGIA
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IMMUNOLOGY
Immune cells: B and T lymphocytes, monocytes, macrophages, dendritic cells, natural killer. Innate immunity, chemical-physical barriers, PAMPs receptors and opsonins, phagocytosis. The antibody mediated response, immunoglobulins, B cell receptor. The complement system, T cell receptor and MHC complex. Immediate, antibody, immunocomplex and cell mediated hypersensitivity. Blood cells: morphology of of eritrocytes, platlets, neutrophil, eosinophil and basophil granulocytes, lymphocytes and monocytes. Practical course staining of blood samples with May Grunewald Giemsa and observation at the microscope of blood cells.
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Modulo: 020135/1 - EMATOLOGIA
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HEMATOLOGY
Blood, Circulation, Composition and functions, CBC and leukocyte formula, cytology and cell functions.
Outline of coagulation pathophysiology (von Willebrandt disease and haemophilia, disseminated intravascular coagulation)
The alterations of the red blood cells (Anemia and polyglobulias);
Alterations of white blood cells (leukocytosis and leukopenias, acute and chronic leukemias, monoclonal lymphomas and gammopathies, chronic myeloproliferative neoplasms, myelodysplastic syndromes);
Alterations in platelets (platelets and platelet disorders)
Each pathology will be treated with a view to laboratory tests and techniques useful for the diagnosis and monitoring of any treatment
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Modulo: 53753 - TECNICHE DI IMMUNOEMATOLOGIA
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TECHNIQUES OF IMMUNOHEMATOLOGY
Red blood cell antigens: biochemistry, genetics, structure and function. Blood groups: ABO, (ABO, Ii, Hh, SEse, Lu, P). RH (Rh weak, Rh null, Rh partial), Kell, Duffy, Kidd and MNSs. Anti red blood cell antibodies. Antigen-antibody binding interactions. The complement and immunohematologic reactions. Direct and indirect antiglobulin test. Intra and extravascular hemolysis. Immune haemolytic anemia (IgG and IgM). Newborn haemolytic anemia. Identification of anti red blood cell antibodies. Cross matching procedures. Transfusion errors and their prevention.
Collection and handling of blood. Reference ranges and normal values. Basic manual and automated hematological techniques for blood cell counting. Preparation and staining methods for blood and bone marrow films. Bone marrow biopsy. Blood cell morphology. Hematopoietic stem cell isolation and characterization. Flow cytometry and immunophenotyping. Didactic methods
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Modulo: 012936/1 - IMMUNOLOGIA
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Lectures and laboratory
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Modulo: 020135/1 - EMATOLOGIA
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HEMATOLOGY
Frontal lessons, using power point presentations and video-clips related to the specific topics day by day. I
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Modulo: 53753 - TECNICHE DI IMMUNOEMATOLOGIA
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Lectures and laboratory Learning assessment procedures
- IMMUNOLOGY: written exam with 36 multiple choice questions (4 possible answers, only 1 correct). Each correct answer corresponds to 1 point, without penalty for uncorrect answers. To pass the exam the students need to respond correctly to at list 18 questions. Examination time (60 min);
HEMATOLOGY: Oral examination, 15-20 minutes in length. The final score will be assigned based on the completeness and clarity of presentation and the confidence on the technical aspects. The maximum score is 30 and honors; the minimum score to pass the exam is 18/30 points
TECHNIQUES OF IMMUNOHEMATOLOGY: Oral examination, 15-20 minutes in length. The final score will be assigned based on the completeness and clarity of presentation and the confidence on the technical aspects. The maximum score is 30 and honors; the minimum score to pass the exam is 18/30 points
FINAL GRADUATION is calculated as arithmetic mean of the three partial marks Reference texts
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Modulo: 012936/1 - IMMUNOLOGIA
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IMMUNOLOGY
Doan et al., Le basi dell’immunologia, con approfondimenti clinici, Zanichelli Ed.
Regueiro Gonzalez et al., Immunologia. Biologia e Patologia del sistema immunitario. Piccin Ed.
Lectures slides
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Modulo: 020135/1 - EMATOLOGIA
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HEMATOLOGY
Gianluigi Castoldi & Vincenzo Liso. Core Curriculum. Ematologia. McGraw-Hill 2ed. 2014
Dacie and Lewis Practical Haematology. 12th edition, Elsevier, Churchill Livingstone.
Cuneo A, Castoldi GL, Rigolin GM. Tecniche di laboratorio nella diagnostica delle emopatie. Trattato italiano di medicina di laboratorio. (fondato da Angelo Burlina). Volume V/1. Piccin Nuova Libraria Padova 2012
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Modulo: 53753 - TECNICHE DI IMMUNOEMATOLOGIA
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TECHNIQUES OF IMMUNOHEMATOLOGY
Slides of the lectures.
Dacie and Lewis Practical Haematology. 11th edition 2011, Elsevier, Churchill Livingstone.
Cuneo A, Castoldi GL, Rigolin GM. Tecniche di laboratorio nella diagnostica delle emopatie. Trattato italiano di medicina di laboratorio. (fondato da Angelo Burlina). Volume V/1. Piccin Nuova Libraria Padova 2012
